Wide Range of Accessories Available for Additional Productivity
The Burleigh® PCS-6000 and Burleigh® PCS-5000 Piezoelectric micromanipulator systems are compatible with a wide range of accessories, providing maximum flexibility and easy customization.
- Burleigh® PCS-500-SSH Steep/Shallow Headstage Adapter
- Pipette Holder Mount
- Extension Bracket
- Magnetic Base
ISO 9001:2000 Registration
Burleigh® PCS-500-SSH Steep/Shallow Headstage Adapter
Enhancing Patch Clamp Productivity
The Burleigh® PCS-500-SSH Steep/Shallow Headstage Adapter adds unprecedented flexibility for positioning the headstage. It allows the orientation of the headstage and pipette holder at very steep or shallow angles (1). And it further simplifies pipette exchange (2) even when the pipette is in the tightest places. Its symmetrical design offers these capabilities from both the left and right side of the microscope.
When working with a pipette close to the condenser or objective of the microscope, swinging the micromanipulator up and out by hand can be a challenge. The Burleigh® PCS-500-SSH Steep/Shallow Headstage Bracket integrates a quick and efficient mechanism that allows you to retract the pipette up to three inches from the preparation, and then easily return to home position after pipette exchange.
Steep pipette angles are often desired to minimize the length of the electrode in solution with the typical angle being greater than 45 degrees. The shorter the length of the pipette in solution, the lower the stray capacitance and the lower the electrical noise measure while recording. On a typical inverted microscope, the maximum approach angle is limited by the interference of the headstage with the condenser. The Steep/Shallow Bracket holds the headstage so that the clearance with the condenser is maximized.
Shallow pipette angles are typically needed when working with brain slices on an upright, fixed stage microscope. The optimum angle allows an electrode to be moved in a straight line over the brain slice chamber and under the microscope objective without breaking the glass pipette. The typical angle is 15 to 25 degrees. The Steep/Shallow Headstage Bracket extends the headstage closer to the brain slice chamber and away from the manipulator. This extended design also makes it easier to arrange several patch manipulators around one brain slice.
The manual linear slide of this bracket, having three inches of travel, allows the pipette to be easily removed from the preparation and a new pipette quickly and easily inserted, without losing "home place". The slide has repeatable stops at both ends of travel and is easily locked by rotating the locking handle. The location of the end-stop is set by moving the dovetail-mounted base of the slide. Using this dovetail mount, the extended location of the headstage and pipette can be set at any distance in only a few seconds. To exchange pipettes, you first simply slide the headstage and pipette back using the linear slide on the Steep/Shallow Headstage Bracket. The next step is to rotate the micromanipulator base out using the base pivot. You then change the pipette, rotate the base pivot back to the repeatable stop and slide the headstage back to home position.
|Holds the headstage||Allows clearance with the condenser to be maximized|
|Extends the headstage closer to the brain slice chamber and away from the manipulator||Provides more flexibility on manipulator location; facilitates positioning of several manipulators around one brain slice|
|Steep angle||Place the minimum amount of pipette tip into the cell|
|Shallow angle||Allows the pipette tip to be very close to the surface of the cell|
|Quick and easy pipette exchange||Aids in your productivity|
MIS-PHM Pipette Holder Mount
The MIS-PHM Pipette Holder Mount accepts pipette holders up to 4mm in diameter. It features a moveable rail that allows the pipette to be easily positioned in a variety of locations. The rotational freedom of the pipette holder clamp assembly allows you to achieve both steep and shallow angles, depending on your needs. Back to Top
Even though the Burleigh micromanipulator systems are designed for maximum flexibility, there may be a time when you need to give it a little boost to achieve perfect pipette position. In this case, you can use the PCS-500-EB Extension Bracket Kit. The kit includes three spacer discs that are three inches in diameter and one-half inch thick. The extension plate is fastened to the mounting surface with one or all of the discs. Back to Top
Can be used to mount to a magnetic surface. This allows quick attachment and detachment of the micromanipulator for more versatile positioning on a magnetic surface. Back to Top