Cultured cells or cells in a monolayer are most often viewed using an inverted microscope. In these instances, the best manipulator is one that has orthogonal axes in XYZ and can provide a steep angle for the tip. For this application, the Burleigh® PCS-6000 Piezoelectric micromanipulator is the recommended product.
Burleigh® PCS-500 Mount fixes directly to your inverted microscope allowing the standard microscope XY stage to move freely beneath it while supporting the Burleigh® PCS-6200 manipulator in the cultured slice configuration (C-configuration). For longer piezo travel we also offer the Burleigh® PCS-6300 and Burleigh® PCS-6400 models.
In general, the recommended equipment configuration for cultured cell recording would be:
|25mm of motorized motion in three linear axes||Coarse positioning without touching the manipulator|
|150 µm of piezo motion in all three axes||Piezo motion provides ultra smooth positioning, essentially turning finger movements into nanometer precision|
|Drift specification of better than 1 µm per hour||Stable patching, providing results faster and with less frustration|
|Easy tip exchange made possible by two rotary stages with adjustable stops||Tip exchange can be accomplished rapidly and easily, in some cases saving as much as 90 minutes of recording time per day|
|XYZ axis||Position the tip over the cell with X and Y and patch using the Z-axis|
|Two year warranty||Peace of mind|