Patch clamping of brain slices is most often performed on an upright microscope and requires a micromanipulator system to have a shallow approach angle of less than 20 degrees. The Burleigh® Gibraltar® Platform is particularly ideal for recording from brain cells.
The Burleigh® Gibraltar® Platform supports the upright microscope and the Burleigh® PCS-6200 Piezoelectric manipulator in the traditional brain slice configuration (T-configuration) with a real approach axis. For longer piezo travel, we also offer the Burleigh® PCS-6300 and Burleigh® PCS-6400 models.
In general, the recommended equipment configuration for brain slice recording would be:
- Gibraltar® Platform
- PCS-6200-T (Dual PCS-6200-T also available)
- PCS-500-SSH (steep shallow head adaptor); for shallow angles
Motorized Burleigh® Gibraltar®
|25 mm of vibration free XY motorized positioning of the microscope||The entire upright microscope can be easily moved, while the brain slice and the manipulator remain stationary|
|Two XY memory positions||Two areas can be reproducibly located increasing efficiency for double patching, time lapse photography|
|Aluminum or stainless steel top||Aluminum platform with through-holes for ease of mounting manipulators or a stainless steel top with tapped holes and spill-proof base|
|25 mm of motorized motion in three linear axes||Coarse positioning without touching the manipulator|
|150 µm of piezo motion in all three axes||Piezo motion provides ultra smooth positioning, essentially turning finger movements into nanometer precision|
|Drift specification of better than 1 µm per hour||Stable patching, providing results faster and with less frustration|
|Easy tip exchange made possible by two rotary stages with adjustable stops||Tip exchange can be accomplished rapidly and easily, in some cases saving as much as 90 minutes of recording time per day|
|A real approach axis||Providing smoother penetration into the brain slice and reducing the possibility of tissue stress|
|Two year warranty||Peace of mind|